Molecular Biologist Toolkit: Restriction Enzymes
A staple in any DIY biologists' toolkit is the restriction enzyme. These nifty proteins can be used to cut DNA at specific sequences of bases producing either blunt or "sticky" ends. When the "sticky ends" are complementary the DNA combines and can result in a new sequence. Knowing how to use restriction enzymes is the first step in synthetic biology.
During our third and final workshop in the Lab Skills 101 Series, participants utilized techniques gained from the previous two classes and added one more skill to their molecular toolkit. Participates used three common restriction enzymes (EcoRI, BamHI, HindIII) to cut bacteriophage DNA. To assess if their cuts were successful, we ran gels to see the band patterns. It was really fun to observe positive outcomes with successful digests!
- Jaylin and Regina